Or is it an item you can only use once on a wolf?

2 - If you use the warrior's stone on an alpha male, he won't be able to breed, ever, that's clear. But what happens to the alpha female? Will she be unable to breed at all (unless replaced with another female), or can she be bred outside the pack like beta/omega females?

1 - Yeah, I believe you can use multiple ones~

2 - I'd assume she wouldn't be able to breed to the alpha male, but if she is demoted to Beta or Omega I would guess that she would be able to breed outside the pack normally. And, similarly, she would probably be able to breed if the Warrior Stone male was replaced with a different alpha male.

However, @Dark Paw Pack, I don't think she would be able to breed to the next highest dominance male as an alpha.